Pet urine can leave behind a lingering odor and stain on your subfloor, but with the right cleaning methods, you can remove the stain and smell completely. Here's a step-by-step guide on how to clean subfloor from pet urine:

How to Clean Subfloor from Pet Urine

Remove Excess Urine

1. Blot Up the Urine: - Use paper towels or a clean cloth to absorb as much urine as possible. - Blot, don't rub, to prevent the urine from spreading.

2. Rinse the Area: - Rinse the area with clean water to dilute the urine. - Use a bucket or a hose with a gentle spray setting.

Clean the Stain

1. Use an Enzyme Cleaner: - Purchase an enzymatic pet urine cleaner from a pet store or online. - Follow the instructions on the product label for mixing and application.

2. Apply the Cleaner: - Apply the enzyme cleaner to the urine stain. - Use a spray bottle or a sponge to apply the cleaner.

3. Let the Cleaner Sit: - Allow the enzyme cleaner to sit on the stain for the recommended amount of time. - This typically ranges from 15 minutes to several hours.

4. Rinse and Dry: - Rinse the area thoroughly with clean water. - Use a wet-dry vacuum to extract as much water as possible.

Neutralize the Odor

1. Use a Baking Soda Paste: - Create a paste by mixing baking soda with a small amount of water. - Apply the paste to the urine stain.

2. Let the Paste Dry: - Allow the baking soda paste to dry completely. - This may take several hours or overnight.

3. Vacuum the Baking Soda: - Vacuum up the dried baking soda paste. - Repeat the process if necessary.

Prevent Future Accidents

1. Train Your Pet: - Train your pet to use a designated area for elimination. - Use positive reinforcement to encourage your pet to use the designated area.

2. Clean Up Accidents Immediately: - If your pet does have an accident, clean it up immediately using the steps outlined above. - Promptly cleaning up accidents will prevent the urine from seeping into the subfloor.

3. Use a Waterproof Barrier: - Consider installing a waterproof barrier, such as a plastic sheet or a sealant, under your subfloor. - This will create a protective layer that prevents urine from penetrating the subfloor.

By following these steps, you can effectively clean subfloor from pet urine and eliminate both the stain and the odor. Remember to train your pet to use a designated area for elimination and to clean up accidents immediately to prevent future issues.